您好，欢迎访问重庆市农业科学院 机构知识库！

Establishment of a Sensitive and Reliable Droplet Digital PCR Assay for the Detection of Bursaphelenchus xylophilus

文献类型： 外文期刊

作者： Su, Yu 1 ; Zhu, Xuedong 2 ; Jing, Haozheng 2 ; Yu, Haiying 3 ; Liu, Huai 1 ;

作者机构： 1.Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China

2.Southeast Chongqing Acad Agr Sci, Chongqing 408000, Peoples R China

3.Natl Forestry & Grassland Adm, Gen Stn Forest & Grassland Pest Management, Shenyang 110034, Peoples R China

关键词： Bursaphelenchus xylophilus determination; nuclear DNA; droplet digital PCR

期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 19 期

页码：

收录情况： SCI

摘要： Pine wilt disease (PWD), which poses a significant risk to pine plantations across the globe, is caused by the pathogenic agent Bursaphelenchus xylophilus, also referred to as the pine wood nematode (PWN). A droplet digital PCR (ddPCR) assay was developed for the quick identification of the PWN in order to improve detection sensitivity. The research findings indicate that the ddPCR assay demonstrated significantly higher analysis sensitivity and detection sensitivity in comparison to traditional quantitative PCR (qPCR). However, it had a more limited dynamic range. High specificity was shown by both the ddPCR and qPCR techniques in the diagnosis of the PWN. Assessments of reproducibility revealed that ddPCR had lower coefficients of variation at every template concentration. Inhibition tests showed that ddPCR was less susceptible to inhibitors. There was a strong linear association between standard template measurements obtained using ddPCR and qPCR (Pearson correlation = 0.9317; p < 0.001). Likewise, there was strong agreement (Pearson correlation = 0.9348; p < 0.001) between ddPCR and qPCR measurements in the evaluation of pine wood samples. Additionally, wood samples from symptomatic (100% versus 86.67%) and asymptomatic (31.43% versus 2.9%) pine trees were diagnosed with greater detection rates using ddPCR. This study's conclusions highlight the advantages of the ddPCR assay over qPCR for the quantitative detection of the PWN. This method has a lot of potential for ecological research on PWD and use in quarantines.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：重庆市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台