Exploring regulatory network of icariin synthesis in Herba Epimedii through integrated omics analysis
文献类型： 外文期刊
作者： Zhu, Xuedong 1 ; Wen, Shiqi 2 ; Gul, Hameed 3 ; Xu, Pan 1 ; Yang, Yang 2 ; Liao, Ximei 2 ; Ye, Yunling 2 ; Xu, Zijian 2 ; Zhang, Xiaofang 1 ; Wu, Lin 1 ;
作者机构： 1.Southwest Univ, Fuling Acad Southwest Univ, Southeast Chongqing Acad Agr Sci, Chongqing, Peoples R China
2.Southwest Univ, Integrat Sci Ctr, Germplasm Creat Western China CHONGQING Sci City, Chongqing, Peoples R China
3.Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China
4.Southwest Univ, Chongqing Key Lab Biol & Genet Breeding Tuber & Ro, Chongqing, Peoples R China
5.Minist Agr & Rural Affairs, Key Lab Germplasm Innovat Upper Yangtze River, Chongqing, Peoples R China
关键词： Epipremnum sagittatum; Epipremnum pubescens; icariin biosynthesis; multi omics analysis; regulatory network
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.3 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Herba Epimedii's leaves are highly valued in traditional Chinese medicine for their substantial concentration of flavonoids, which play a crucial role in manifesting the plant's therapeutic properties. This study investigated the metabolomic, transcriptomic and proteomic profiles of leaves from two Herba Epimedii cultivars, Epipremnum sagittatum (J) and Epipremnum pubescens (R), at three different developmental stages. Metabolite identification and analysis revealed a total of 1,412 and 1,421 metabolites with known structures were found. Flavonoids made up of 33%, including 10 significant accumulated icariin analogues. Transcriptomic analysis unveiled totally 41,644 differentially expressed genes (DEGs) containing five encoded genes participated in icariin biosynthesis pathways. Totally, 9,745 differentially expressed proteins (DEPs) were found, including Cluster-47248.2.p1 (UDP-glucuronosy/UDP-glucosyltransferase), Cluster-30441.2.p1 (O-glucosyltransferase), and Cluster-28344.9.p1 (anthocyanidin 3-O-glucoside 2 "-O-glucosyltransferase-like) through proteomics analysis which are involved to icariin biosynthesis. Protein-protein interaction (PPI) assay exhibited, totally 12 proteins showing a strong relationship of false discovery rate (FDR) <0.05 with these three proteins containing 2 leucine-rich repeat receptor kinase-like protein SRF7, and 5 methyl jasmonate esterase 1. Multi-omics connection networks uncovered 237 DEGs and 72 DEPs exhibited significant associations with the 10 icariin analogues. Overall, our integrated omics approach provides comprehensive insights into the regulatory network underlying icariin synthesis in Herba Epimedii, offering valuable resources for further research and development in medicinal plant cultivation and pharmaceutical applications.
- 相关文献
作者其他论文 更多>>
-
Establishment of a Sensitive and Reliable Droplet Digital PCR Assay for the Detection of Bursaphelenchus xylophilus
作者：Su, Yu;Liu, Huai;Su, Yu;Zhu, Xuedong;Jing, Haozheng;Yu, Haiying
关键词：Bursaphelenchus xylophilus determination; nuclear DNA; droplet digital PCR
-
Antifungal activity and mechanism of chitosan against Fusarium solani caused ginger soft rot during postharvest storage
作者：Zhang, Lingling;Yang, Ying;Zhu, Yongxing;Hu, Haijun;Jia, Qie;Sun, Chong;Liu, Yiqing;Sun, Chong;Liu, Yiqing;Zhu, Xuedong
关键词：Chitosan; Antifungal; Fusarium solani; Ginger; Postharvest
-
Eggplant NAC domain transcription factor SmNST1 as an activator promotes secondary cell wall thickening
作者：Wang, Jiali;Zhang, Xinxin;Yang, Huiqin;Li, Sirui;Hu, Yao;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Wang, Jiali;Zhang, Xinxin;Yang, Huiqin;Li, Sirui;Hu, Yao;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Yang, Yang;Tian, Shibing
关键词：NAC transcription factors; SCW biosynthesis; SmMYB108; SmMYB26; SmNST2
-
The regulatory roles of MYC TFs in plant stamen development
作者：Li, Sirui;Hu, Yao;Yang, Huiqin;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Li, Sirui;Hu, Yao;Yang, Huiqin;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Tian, Shibing;Yang, Yang
关键词：Anther; Jasmonate; MYC transcription factor; Phytohormone; Pollen; Stamen development
-
Comparative transcriptome analysis reveals the involvement of an MYB transcriptional activator, SmMYB108, in anther dehiscence in eggplant
作者：Hu, Ruolin;Wang, Jiali;Yang, Huiqing;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Hu, Ruolin;Wang, Jiali;Yang, Huiqing;Wei, Dayong;Tang, Qinglin;Wang, Zhimin;Yang, Yang;Tian, Shibing
关键词：eggplant; MYB transcription factor; anther dehiscence; regulation of gene expression; RNA-seq
-
The molecular mechanism of eggplant parthenocarpy revealed through a combined analysis of the transcriptome and the metabolome
作者：Zhou, Shanshan;Yang, Yang;Zou, Min;Tao, Tao;Wang, Yongqing;Tian, Shibing;Tang, Xiaohua
关键词：Eggplant; Parthenocarpy; RNA-seq; Metabolome
-
Integrated analyses of metabolomics and transcriptomics reveal the potential regulatory roles of long non-coding RNAs in gingerol biosynthesis
作者：Zhang, Wenlin;Li, Honglei;Li, Zhexin;Liao, Qinhong;Tang, Jianmin;Zhang, Wenlin;Zhao, Guohua;Yang, Yang;Yang, Suyu;Liao, Ximei;Wu, Lin;Zhu, Xuedong
关键词：Ginger; Metabolomics; Transcriptomics; Long non-coding RNAs; Gingerol biosynthesis
