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The Catalase Gene MrCat1 Contributes to Oxidative Stress Tolerance, Microsclerotia Formation, and Virulence in the Entomopathogenic Fungus Metarhizium rileyi

文献类型： 外文期刊

作者： Su, Yu 1 ; Wang, Xuyi 2 ; Luo, Yuanli 2 ; Jiang, Huan 2 ; Tang, Guiting 2 ; Liu, Huai 1 ;

作者机构： 1.Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China

2.Southeast Chongqing Acad Agr Sci, Chongqing 408000, Peoples R China

关键词： Metarhizium rileyi; catalase; oxidative stress tolerance; microsclerotium; virulence

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Catalases play a crucial role in the metabolism of reactive oxygen species (ROS) by converting H2O2 into molecular oxygen and water. They also contribute to virulence and fungal responses to various stresses. Previously, the MrCat1-deletion mutant (Delta MrCat1) was generated using the split-marker method in Metarhizium rileyi. In this study, the Cat1 gene was identified, and its function was evaluated. Under normal culture conditions, there were no significant differences in colony growth or dimorphic switching between Delta MrCat1 and the wild-type (WT) strains. However, under oxidative stress, the colony growth was inhibited, and the yeast-hyphal transition was suppressed in the Delta MrCat1 strain. Hyperosmotic stress did not differ significantly between the two strains. In the Delta MrCat1 strain, microsclerotia (MS) formation was delayed, resulting in less uniform MS size and a 76% decrease in MS yield compared to the WT strain. Moreover, the Delta MrCat1 strain exhibited diminished virulence. Gene expression analysis revealed up-regulation of Delta MrCat1, MrCat2, MrCat4, and MrAox in the Delta MrCat1 strain. These findings indicate that the MrCat1 gene in M. rileyi is essential for oxidative stress tolerance, MS formation, and virulence.

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