您好，欢迎访问重庆市农业科学院 机构知识库！

Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant

文献类型： 外文期刊

作者： Zhang, Yanjie 1 ; Chu, Guihua 1 ; Hu, Zongli 1 ; Gao, Qiong 1 ; Cui, Baolu 1 ; Tian, Shibing 2 ; Wang, Bo 3 ; Chen, Guopi 1 ;

作者机构： 1.Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, Bioengn Coll, Campus B,Room 515,174 Shapingba Main St, Chongqing 400044, Peoples R China

2.Chongqing Acad Agr Sci, Inst Vegetable Res, Chongqing 401329, Peoples R China

3.Chongqing Acad Agr Sci, Inst Vegetable

关键词： Eggplant;SmMYB1;Anthocyanin accumulation;HPLC-ESI-MS/MS;Transcriptional regulation;Freezing stress

期刊名称：MOLECULAR BREEDING （ 影响因子：2.6； 五年影响因子：2.7 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Anthocyanins, natural pigments with high antioxidant activities, are widely distributed in the plant kingdom and play important roles in various physiological processes. Much effort has been committed to enhancing the anthocyanin content of these health-promoting pigments in vegetables and grains, for their eye-catching colors and special nutrients. Previously, we reported that the SmMYB1 gene encoding a R2R3 MYB transcription factor participated in the regulation of anthocyanin biosynthesis in the peel of eggplant. In this work, we introduced SmMYB1 into a non-anthocyanin-accumulating egg-plant cultivar (Solanum aethiopicum group Gilo) via Agrobacterium-mediated transformation. Genetically engineered plants exhibited high concentrations of anthocyanin in leaves, petals, stamens, and fruit peels under normal growth conditions, especially in fruit flesh. Furthermore, highly methylated anthocyanins, malvidin 3-(p-coumaroyl) rhamnoside(glucoside)-5-glucoside and malvidin 3-(feruloyl)rhamnoside(glucoside)-5-glucoside, were separated from the purple fruit flesh and identified by HPLC-ESI-MS/MS. qRT-PCR analysis revealed that most anthocyanin structural genes were dramatically up-regulated in the tissues of transgenic lines compared with non-transformed plants. In addition, the transgenic seedlings had greater tolerance to freezing stress and better recovery under rewarming conditions. These results provide a good foundation for the breeding of new eggplant cultivars with more healthy agronomic traits in future studies.

  • 相关文献

[1]Anthocyanin Accumulation and Molecular Analysis of Anthocyanin Biosynthesis-Associated Genes in Eggplant (Solanum melongena L.). Zhang, Yanjie,Hu, Zongli,Chu, Guihua,Huang, Cheng,Zhao, Zhiping,Chen, Guoping,Tian, Shibing. 2014

[2]Anthocyanin Accumulation and Molecular Analysis of Correlated Genes in Purple Kohlrabi (Brassica oleracea var. gongylodes L.). Zhang, Yanjie,Hu, Zongli,Zhu, Mingku,Zhu, Zhiguo,Chen, Guoping,Wang, Zhijin,Tian, Shibing.

[3]Accumulation and Molecular Regulation of Anthocyanin in Purple Tumorous Stem Mustard (Brassica juncea var. tumida Tsen et Lee). Xie, Qiaoli,Hu, Zongli,Zhang, Yanjie,Zhao, Zhiping,Chen, Guoping,Tian, Shibing,Wang, Zhijin,Yang, Yang.

[4]Anthocyanin Accumulation and Transcriptional Regulation of Anthocyanin Biosynthesis in Purple Bok-Choy (Brassica rapa var. chinensis). Zhang, Yanjie,Chen, Guoping,Dong, Tingting,Pan, Yu,Zhao, Zhiping,Hu, Zongli,Tian, Shibing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：重庆市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台