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Genome-Wide Identification and Expression Analysis of Hexokinase Gene Family Under Abiotic Stress in Tomato

文献类型： 外文期刊

作者： Li, Jing 1 ; Yao, Xiong 2 ; Zhang, Jianling 3 ; Li, Maoyu 4 ; Xie, Qiaoli 1 ; Yang, Yingwu 1 ; Chen, Guoping 1 ; Zhang, Xianwei 2 ; Hu, Zongli 1 ;

作者机构： 1.Chongqing Univ, Bioengn Coll, Lab Mol Biol Tomato, Room 523-1,Campus B,174 Shapingba Main St, Chongqing 400030, Peoples R China

2.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China

3.Liaocheng Univ, Coll Agr & Biol, Lab Plant Germplasm Resources Innovat & Utilizat, Liaocheng 252000, Peoples R China

4.Chongqing Seed Stn, Chongqing 401121, Peoples R China

关键词： tomato; hexokinase; molecular characterization; expression pattern; abiotic stress

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 3 期

页码：

收录情况： SCI

摘要： In plants, hexokinase (HXK) is a kind of bifunctional enzyme involved in sugar metabolism and sugar signal transduction that plays important roles in plant growth and development and stress response. Some HXK genes without a phosphorylation function have been found in Arabidopsis, tobacco, etc., but these genes have not been identified in tomato. Therefore, further genome-wide systematic identification and characterization is necessary for tomato HXK genes. In this study, six HXK genes were identified from the tomato genome distributed across six different chromosomes, named SlHXK1-6. Gene structure analysis showed that the SlHXK genes contain the same number of introns and exons. Gene duplication and collinearity analysis revealed two pairs of tandem repeats among SlHXKs, and a higher collinearity between tomatoes and potatoes were found. Response elements associated with phytohormones, abiotic stresses, and growth and development were identified in the promoter sequences of SlHXKs. Quantitative real-time PCR (qRT-PCR) results further indicated the potential role of SlHXKs in tomato development and stress responses. The expression levels of most SlHXKs were significantly induced by abiotic stress, hormone, and sugar solution treatments. In particular, the expression of SlHXK1 was significantly induced by various treatments. Functional complementation experiments were performed using HXK-deficient yeast strain YSH7.4-3C (hxk1, hxk2, and glk1), and the results showed that SlHXK5 and SlHXK6 were unable to phosphorylate glucose and fructose in yeast. In conclusion, these results provide valuable foundations for further exploring the sugar metabolism and sugar signal transduction mechanisms of HXK and the functions of SlHXK genes in various abiotic stresses, and some SlHXKs may be key genes for enhancing plants' tolerance to abiotic stresses.

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