Anthocyanin Accumulation and Molecular Analysis of Correlated Genes in Purple Kohlrabi (Brassica oleracea var. gongylodes L.)
文献类型： 外文期刊
作者： Zhang, Yanjie 1 ; Hu, Zongli 1 ; Zhu, Mingku 1 ; Zhu, Zhiguo 1 ; Wang, Zhijin 2 ; Tian, Shibing 2 ; Chen, Guoping 1 ;
作者机构： 1.Chongqing Univ, Minist Educ, Bioengn Coll, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China
2.Chongqing Acad Agr Sci, Inst Vegetable Res, Chongqing 401329, Peoples R China
关键词： anthocyanin accumulation;purple kohlrabi;structural genes;transcription factors;HPLC-ESI-MS/MS;Brassica oleracea var. gongylodes L
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.7； 五年影响因子：6.0 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Kohlrabi (Brassica oleracea var. gongylodes L.) is an important dietary vegetable cultivated and consumed widely for the round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation in the leaf and swollen stem. Here, different kinds of anthocyanins were separated and identified from the purple kohlrabi cultivar (Kolibri) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. In order to study the molecular mechanism of anthocyanin biosynthesis in purple kohlrabi, the expression of anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi and a green cultivar (Winner) was examined by quantitative PCR. In comparison with the colorless parts in the two cultivars, most of the anthocyanin biosynthetic genes and two transcription factors were drastically upregulated in the purple tissues. To study the effects of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin contents and transcripts of associated genes were analyzed in sprouts of both cultivars grown under light and dark conditions.
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