文献类型： 外文期刊
作者： Wang, Huimin 1 ; Xiang, Tingsheng 2 ; Wang, Yanwei 1 ; Song, Jinlong 3 ; Zhai, Yi 4 ; Chen, Xiaorong 1 ; Li, Yanting 1 ;
作者机构： 1.CAAS, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources, Beijing 100081, Peoples R China
2.Yangtze Univ, Earth Environm & Water Resources Coll, Jinzhou 434023, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
4.Chongqing Acad Agr Sci, Inst Agr Engn, Chongqing 401329, Peo
期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）
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收录情况： SCI
摘要： A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410(T), was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and.pH ranges for growth were 4 degrees C to 50 degrees C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12% (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C-15:0, anteiso-C-17:0 and iso-C-16:0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153(T) and Microbacterium murale DSM 22178(T) with 97.50/o and 97.4% sequence similarities, respectively; while the DNA DNA hybridization values were 33.1 +/- 3.4 % and 21.8 +/- 1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T-m method. Based on its phenotypic and genotypic properties, strain LAM0410(T) represents a novel species of the genus.Microbacterium, for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410(T) (=ACCC 00719(T)=JCM 19612(T)).
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