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Clostridium huakuii sp nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia

文献类型： 外文期刊

作者： Ruan, Zhiyong 1 ; Wang, Yanwei 1 ; Zhang, Chi 2 ; Song, Jinlong 3 ; Zhai, Yi; Zhuang, Yan 1 ; Wang, Huimin 1 ; Chen, X 1 ;

作者机构： 1.CAAS, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources, Beijing 100081, Peoples R China

2.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

3.Chinese Aca

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

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收录情况： SCI

摘要： A Gram-staining-positive, spore-forming, obligately anaerobic, acetogenic bacterium, designated LAM1030(T), was isolated from methanogenic consortia enriched from biogas slurry collected from the large-scale anaerobic digester of Modern Farming Corporation in Hebei Province, China. Cells of strain LAM1030(T) were motile, straight or spiral-rod-shaped. Strain LAM1030(T) could utilize glucose, fructose, maltose, galactose, lactose, sucrose, cellobiose, mannitol, pyruvate, succinic acid and tryptophan as the sole carbon source. Acetic acid, isovaleric acid and butanoic acid were the main products of glucose fermentation. Sodium sulfite was used as an electron acceptor. Growth of strain LAM1030(T) was completely inhibited by the addition of ampicillin, tetracycline, gentamicin or erythromycin at a concentration of 20 mu g ml(-1). The main polar lipids of strain LAM1030(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, 11 unknown glycolipids and two unknown phospholipids. No respiratory quinone was detected. The major fatty acids of strain LAM1030(T) were C-16:0 (21.1 %), C-14:0 (10.3 %), summed feature 9 (including C-16:0 10-methyl and/or iso-C-17:1 omega 9c) (11.3%), summed feature 3 (including C-16:1 omega 7c and/or C-16:1 co6c) (10.6%) and iso-C-15:0 (6.6%). Analysis of the 16S rRNA gene sequence indicated that strain LAM10301- belonged to the genus Clostridium and was most closely related to Clostridium subterminale DSM 69701, Clostridium thiosulfatireducens DSM 13105(T) and Clostridium sulfidigenes DSM 18982(T), with 97.0, 96.9 and 96.8% similarity, respectively. The G+C content of the genomic DNA of strain LAM1030(T) was 31.2 +/- 0.3 molok. On the basis of its phenotypic, phylogenetic and chemotaxonomic characterization, strain LAM1030(T) is suggested to represent a novel species of the genus Clostridium, for which the name Clostridium huakuii sp. nov. is proposed. The type strain is LAM1030(T) (=ACCC 00698(T)=JCM 19186(T)).

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