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Dissection of the genetic architecture of seed erucic acid content and consequences for breeding in Brassica napus L

文献类型： 外文期刊

作者： Liu, Haijiang 1 ; Yuan, Yongheng 1 ; Ye, Kaijie 1 ; Li, Ruoshui 1 ; Ran, Hu 1 ; Tang, Yunshan 1 ; Lu, Kun 1 ; Yin, Nengwen 1 ; Zhao, Huiyan 1 ; Li, Jiana 1 ; Huang, Taocui 5 ; Shi, Lei 6 ; Du, Hai 1 ; Qu, Cunmin 1 ;

作者机构： 1.Southwest Univ, Integrat Sci Ctr Germplasm Creat Western China CHO, Chongqing, Peoples R China

2.Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China

3.Southwest Univ, Engn Res Ctr South Upland Agr, Minist Educ, Chongqing 400715, Peoples R China

4.Southwest Univ, Acad Agr Sci, Chongqing, Peoples R China

5.Chongqing Acad Agr Sci, Chongqing 40216, Peoples R China

6.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

关键词： Brassica napus; Genome-wide association study; Transcriptome-wide association study; Seed erucic acid content; Haplotype analysis; Molecular marker

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 226 卷

页码：

收录情况： SCI

摘要： Oilseed rape (Brassica napus L.) is an important oilseed crop worldwide. The seed erucic acid content (SEAC) determines the quality of the extracted rapeseed oil, with lower SEAC levels indicating higher quality oil. To elucidate the genetic mechanism underlying SEAC and identify candidate SEAC-regulatory genes, a panel of 393 diverse B. napus accessions was screened in a genome-wide association study (GWAS) using 1.6 million high- quality single-nucleotide polymorphism (SNP) markers. We identified 19 genetic regions significantly associated with SEAC using factored spectrally transformed linear mixed model (FaST-LMM), with the phenotypic variation explained (PVE) ranging from 16.67 % to 47.66 %. Additionally, a transcriptome-wide association study (TWAS) was conducted on the SEAC of this association panel, identifying 366 significantly associated genes. Eight genes were identified in both the GWAS and TWAS, and their expression levels were significantly correlated with the SEAC. A candidate gene association analysis and haplotype analysis revealed several lowSEAC haplotypes, and inbred lines combining the low-SEAC haplotypes had lower SEAC and seed glucosinolate contents but higher unsaturated fatty acids, without affecting the overall seed oil content or seed yield. A molecular marker (Bn_C3_SEAC_Marker) was developed based on the functional SNPs within candidate genes by using the cleaved amplified polymorphic sequence (CAPS) method. The candidate genes, favorable haplotypes, and molecular markers identified in this study will contribute to the cultivation of high-quality B. napus varieties.

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