Genome-wide association study and transcriptome comparison reveal novel QTL and candidate genes that control petal size in rapeseed
文献类型: 外文期刊
作者: Qian, Mingchao 1 ; Fan, Yonghai 1 ; Li, Yanhua 2 ; Liu, Miao 1 ; Sun, Wei 1 ; Duan, Huichun 1 ; Yu, Mengna 1 ; Chang, Wei 1 ;
作者机构: 1.Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China
2.Chongqing Acad Agr Sci, Inst Characterist Crop Res, Chongqing 402160, Peoples R China
3.Southwest Univ, Acad Agr Sci, Chongqing 400715, Peoples R China
4.Minist Educ, Engn Res Ctr South Upland Agr, Chongqing 400715, Peoples R China
关键词: Ethylene signalling; genome-wide association study; petal size; rapeseed; transcriptome
期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:5.6; 五年影响因子:6.8 )
ISSN: 0022-0957
年卷期: 2021 年 72 卷 10 期
页码:
收录情况: SCI
摘要: Petal size determines the value of ornamental plants, and thus their economic value. However, the molecular mechanisms controlling petal size remain unclear in most non-model species. To identify quantitative trait loci and candidate genes controlling petal size in rapeseed (Brassica napus), we performed a genome-wide association study (GWAS) using data from 588 accessions over three consecutive years. We detected 16 significant single nucleotide polymorphisms (SNPs) associated with petal size, with the most significant SNPs located on chromosomes A05 and C06. A combination of GWAS and transcriptomic sequencing based on two accessions with contrasting differences in petal size identified 52 differentially expressed genes (DEGs) that may control petal size variation in rapeseed. In particular, the rapeseed gene BnaA05.RAP2.2, homologous to Arabidopsis RAP2.2, may be critical to the negative control of petal size through the ethylene signaling pathway. In addition, a comparison of petal epidermal cells indicated that petal size differences between the two contrasting accessions were determined mainly by differences in cell number. Finally, we propose a model for the control of petal size in rapeseed through ethylene and cytokinin signaling pathways. Our results provide insights into the genetic mechanisms regulating petal size in flowering plants.
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