Ralstonia pseudosolanacearum anthranilate synthase TrpE displays dual roles in tryptophan biosynthesis and promoting expression of genes for type three secretion system
文献类型： 外文期刊
作者： Meng, Pengfei 1 ; Zang, Jin 1 ; Liang, Tao 3 ; Zhou, Jinggang 4 ; Guo, Tao 1 ; Ohnishi, Kouhei 5 ; Zhang, Yong 1 ;
作者机构： 1.Southwest Univ, Coll Resources & Environm, Chongqing, Peoples R China
2.Southwest Univ, Interdisciplinary Res Ctr Agr Green Dev Yangtze Ri, Chongqing, Peoples R China
3.Chongqing Acad Agr Sci, Chongqing, Peoples R China
4.Chongqing Appraisal Ctr Ecol Environm & Engn, Chongqing, Peoples R China
5.Kochi Univ, Fac Agr & Marine Sci, Nankoku, Japan
期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）
ISSN： 0885-5765
年卷期： 2025 年 136 卷
页码：
收录情况： SCI
摘要： Anthranilate synthase TrpEG, comprising a large subunit (TrpE) and a small subunit (TrpG), controls transformation of chorismic acid into anthranilic acid, the first step of tryptophan biosynthesis following the shikimate pathway in many microorganisms. We demonstrated here that TrpE controls tryptophan biosynthesis following the shikimate pathway in Ralstonia pseudosolanacearum. The trpE mutants grew weakly inside host plants but failed to cause any wilting symptoms on host tomato and tobacco plants. Supplementary tryptophan substantially restored impaired growth of trpE mutants in xylem vessels of hydroponic cultivated tomato cuttings, and trpE mutants were able to wilt partial tryptophan-treated hydroponic cultivated tomato cuttings, indicating that insufficient tryptophan inside tomato plants is an important clue for the diminished pathogenicity. Genes encoding a type three secretion system (T3SS) are pathogenicity determinants of R . pseudosolanacearum and impact of TrpE on expression of the T3SS genes was evaluated. Supplementary tryptophan significantly enhanced expression levels of the T3SS genes both in vitro and in planta. Notably, impact of TrpE on T3SS expression was independent of growth deficiency under nutrient-limited conditions. Involvement of TrpE in the T3SS expression was mediated through key regulator HrpB, but via parallel pathways of PrhJ-HrpG and PrhG to HrpB. These results provide novel insights into biological function of TrpE and intricate regulation of T3SS expression in R. pseudosolanacearum.
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