文献类型： 外文期刊

作者： Shen, Yaou ¹ ; Li, Shujun ² ; Jiang, Zhou ¹ ; Ma, Langlang ¹ ; Lin, Haijian ¹ ; Peng, Huanwei ³ ; Liu, Li ¹ ; Yuan, Guang ¹ ;

作者机构： 1.Sichuan Agr Univ, Maize Res Inst, Key Lab Biol & Genet Improvement Maize Southwest, Minist Agr, Chengdu 611130, Sichuan, Peoples R China

2.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China

3.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China

4.Sichuan Agr Univ, Coll Life & Sci, Yaan 625014, Sichuan, Peoples R China

关键词： maize;Incw2 gene;overexpression in endosperm;Yield-related traits

期刊名称：MAYDICA （ 影响因子：0.4； 五年影响因子：0.6 ）

ISSN： 0025-6153

年卷期： 2012 年 57 卷 1-4 期

页码：

收录情况： SCI

摘要： High yield is an eternal goal for crop breeding. Incw2 protein is the enzyme in the metabolic pathway that mobilizes photoassimilated sucrose into numerous reactions of the developing plant seeds, associated with grain yield. In the research, an Incw2 gene driven by 27 kD zein promoter was specifically over-expressed in the endosperm cells of maize inbred line 18-599R by Agrobacterium-mediated genetic transformation. PCR assay displayed that ten of the regenerated plants were integrated with the target gene. By semi-quantitative RT-PCR and invertase activity analysis, five of them showed significantly higher expression of Incw2 transcripts and enzyme activity compared to the wild type. Among them, line 1 stood out because it possessed the highest level of Incw2 mRNA and enzyme activity. The effects of Incw2 over-expression were reflected in the increased chlorophyll content, improved photosynthesis and delay of leaf senility. In addition, yield-related traits such as ear length, ear diameter, ear weight, grain weight per ear, and hundred-kernel weight appeared to be improved in three of the transformants compared with the wild type. The grain weight per plant of line1 was increased by nearly 10%. The results collectively indicate that it is potentially practical to enhance kernel yield of maize by overexpression of Incw2 in endosperm.