The Draft Genome Sequence of Pseudomonas putida Strain TGRB4, an Aerobic Bacterium Capable of Producing Methylmercury
文献类型： 外文期刊
作者： Xiang, Yuping 1 ; Wang, Yongmin 1 ; Shen, Hong 1 ; Wang, Dingyong 1 ;
作者机构： 1.Southwest Univ, Coll Resources & Environm, Chongqing 400715, Peoples R China
2.Chongqing Engn Res Ctr Agr Nonpoint Source Pollut, Chongqing 400715, Peoples R China
3.Chongqing Key Lab Agr Resources & Environm, Chongqing 400715, Peoples R China
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.3； 五年影响因子：2.4 ）
ISSN： 0343-8651
年卷期： 2020 年 77 卷 4 期
页码：
收录情况： SCI
摘要： Mercury (Hg) methylation is mainly a microbial process mediated by anaerobes. The continued study of Pseudomonas putida (P. putida) strain TGRB4 genome was inspired by the fact that it can transform Hg into the highly toxic methylmercury (MeHg) under aerobic conditions. P. putida strain TGRB4 is a Gram-negative rod-shaped Gamma-proteobacterium (gamma-proteobacterium), isolated from the soil in a typical water level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR), which suffered from seasonally water level alternations every year. Draft genome assembly of P. putida strain TGRB4 is presented here, which was sequenced using Illumina Hiseq and PacBio single-molecule real-time (SMRT) platforms. Its genome harbors a total of 5504 genes and a G + C content of 62.6%. We further identified the enzymes related to Hg methylation, and found two well-known methyltransferase, including 5-methyl-tetrahydrofolate (5-methyl-THF) and S-adenosylmethionine (AdoMet), were annotated in the genome of P. putida strain TGRB4. This genome information could be treated as a research material to further study the Hg methylation mechanisms under aerobic environment.
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