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OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice

文献类型： 外文期刊

作者： Shen, Lan 1 ; Zhang, Qiang 1 ; Wang, Zhongwei 2 ; Wen, Hongling 1 ; Hu, Guanglian 1 ; Ren, Deyong 1 ; Hu, Jiang 1 ; Zhu, 1 ;

作者机构： 1.Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China

2.Chongqing Acad Agr Sci, Biotechnol Res Ctr, Chongqing 401329, Peoples R China

关键词： OsCAF2; CRM domain; Intron splicing; Chloroplast development; Rice

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize andArabidopsis thaliana.However, the research of theOsCAF2gene in rice is very little, and the effects ofOsCAF2genes on chloroplasts development are not well characterized. Results In this study,oscaf2mutants were obtained by editing theOsCAF2gene in theNipponbarevariety of rice. Phenotypic analysis showed that mutations toOsCAF2led to albino leaves at the seeding stage that eventually caused plant death, andoscaf2mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated thatOsCAF2might participate in the splicing of group IIA and IIB introns, which differs from its orthologs inA. thalianaand maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers. Conclusion Taken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice.

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