文献类型： 外文期刊
作者： Peng, Qin 1 ; Luchang, Deng 1 ; Weilan, Chen 1 ; Juan, Huang 3 ; Shijun, Fan 1 ; Bin, Tu 1 ; Jun, Tan 2 ; Hua, Yuan 1 ; Yupi 1 ;
作者机构： 1.Sichuan Agr Univ, Rice Res Inst, State Key Lab Crop Gene Explorat & Utilizat South, Chengdu 611130, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China
3.Chongqing Acad Agr Sci, Characterist Crop Res Inst, Chongqing 402160, Peoples R China
关键词： rice; MS92/PTC1; plant homeodomain finger; cis-element; male sterility; anther
期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）
ISSN： 1672-6308
年卷期： 2020 年 27 卷 5 期
页码：
收录情况： SCI
摘要： Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant (ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.
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