文献类型： 外文期刊

作者： Hu, Shiwen ¹ ; Wang, Pan ³ ; Ke, Jianhong ⁴ ; Hui, Junmin ² ; Wang, Cun ² ; Luo, Jing ¹ ; Chen, Shaocheng ¹ ;

作者机构： 1.Chongqing Univ Educ, Chongqing Field Sci Observat & Res Stn Authent Tra, Three Gorges Reservoir Area, Chongqing 400067, Peoples R China

2.Chongqing Univ Educ, Coll Biol & Chem Engn, Chongqing 400067, Peoples R China

3.Chongqing Univ, Chongqing Emergency Med Ctr, Dept Traumatol, Cent Hosp, Chongqing 400013, Peoples R China

4.Chongqing Acad Agr Sci, Corn Res Inst, Chongqing 401329, Peoples R China

5.Chongqing Univ, Coll Biol & Chem Engn, 9 Xuefu Main St, Chongqing 400067, Peoples R China

关键词： Peucedanum praeruptorum Dunn; oxidative damage; pig renal epithelial LLC-PK1 cells; H2O2

期刊名称：EXPERIMENTAL AND THERAPEUTIC MEDICINE （ 影响因子：2.4； 五年影响因子：2.3 ）

ISSN： 1792-0981

年卷期： 2023 年 26 卷 5 期

页码：

收录情况： SCI

摘要： Peucedanum praeruptorum Dunn extract (PPDE) is a well-known treatment used in traditional Chinese medicines, where it is most commonly used to treat coughs and symptoms such as headaches and fever. In the present study, the antioxidant capacity of PPDE in vitro was determined by scavenging experiments using DPPH, ABTS(+), OH, and O-2(-). The cell survival rate was determined by MTT assay. The MDA, SOD, CAT, GSH, and GSH-Px content were determined by colorimetry assays. The expression levels of antioxidant genes SOD, CAT, GSH, and GSH-Px were assessed by reverse transcription-quantitative PCR. HPLC was used to identify the PPDE components. The results suggested that PPDE had scavenging effects on DPPH, ABTS, hydroxyl, and superoxide anion radicals in a concentration-dependent manner; H2O2 treatment resulted in oxidative stress in LLC-PK1 cells, and the degree of injury of LLC-PK1 cells following PPDE treatment was improved, which was positively correlated with its concentration. Peucedanum praeruptorum Dunn extract treatment reduced the content of MDA and increased the content of CAT, SOD1, GSH, and GSH-Px. The mRNA expression levels of antioxidant genes detected by quantitative PCR were consistent with changes in CAT, SOD, GSS, and GSH-Px. Additionally, the trend in CAT, SOD1, GSH, and GSS protein expression levels was also consistent at the mRNA level. PPDE was found to consist of isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol. Therefore, PPDE, which was formed of products derived from natural substances, functioned in the inhibition of oxidative damage. The present study aimed to obtain a better understanding of the traditional Chinese medicine Peucedanum praeruptorum Dunn and preliminarily elucidate its antioxidant mechanism at the cellular level. Further animal or human experiments are required to verify the antioxidant effects of PPDE for further development and utilization.