文献类型： 外文期刊

作者： Niu, Ying ¹ ; Guo, Jianguo ¹ ; Head, Graham P. ⁴ ; Price, Paula A. ⁴ ; Huang, Fangneng ¹ ;

作者机构： 1.Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA

2.Chongqing Acad Agr Sci, Inst Pomol, Chongqing, Peoples R China

3.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou, Gansu, Peoples R China

4.Bayer Crop Sci, St Louis, MO USA

关键词： Spodoptera frugiperda; transgenic plants; MON 89034; dual-gene resistance; genotype; resistance risk assessment

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2019 年 75 卷 8 期

页码：

收录情况： SCI

摘要： BACKGROUND The fall armyworm, Spodoptera frugiperda (J.E. Smith), is the first target pest that has developed resistance to Bt crops across several countries. Leaf tissue and whole plant assays were employed to determine the survival, development, progeny production, and net reproductive rate of all nine possible genotypes of Cry1A.105/Cry2Ab2-dual gene resistant S. frugiperda on non-Bt and MON 89034 maize expressing the Cry1A.105/Cry2Ab2 proteins. RESULTS The homozygous resistant genotype was highly resistant to Bt plants. Genotypes possessing only Cry2Ab2 resistance alleles (RAs) or two Cry1A.105 RAs only were susceptible to MON 89034 with a functional dominance level (D-ML) of 0.0-0.07 on MON 89034 plants. In contrast, genotypes containing two Cry1A.105 plus one Cry2Ab2 RA performed well on Bt plants, with a D-ML of 0.11-0.78. Significant numbers of survivors on Bt plants were also observed for genotypes containing a single Cry1A.105 RA, or a Cry1A.105 plus one or two Cry2Ab2 RAs, with a D-ML of 0.0-0.47. CONCLUSIONS The fitness of individual resistant genes on pyramided Bt plants varied in the dual-gene resistance system. Genotypes containing more Cry1A.105 RAs performed better than those possessing more Cry2Ab2 RAs. The functional dominance level of an individual resistant gene in this system is related to the D-ML level in its corresponding single-gene system. Data generated from this study should fill gaps in understanding dual-/multiple-gene Bt resistance, as well as providing useful information for refining resistance modeling, improving resistance risk assessment, and developing management strategies for the sustainable use of pyramided Bt maize technology. (c) 2019 Society of Chemical Industry