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Hetero-fertilization together with failed egg sperm cell fusion supports single fertilization involved in in vivo haploid induction in maize

文献类型： 外文期刊

作者： Tian, Xiaolong 1 ; Qin, Yuanxin 1 ; Chen, Baojian 1 ; Liu, Chenxu 1 ; Wang, Lele 1 ; Li, Xingli 1 ; Dong, Xin 1 ; Liu, Li 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Natl Maize Improvement Ctr China, Yuanmingyuan West Rd, Beijing 100193, Peoples R China

2.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China

关键词： Cell fusion; fertilization recovery; haploid; hetero-fertilization; maize; single fertilization

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 0022-0957

年卷期： 2018 年 69 卷 20 期

页码：

收录情况： SCI

摘要： In vivo doubled-haploid technology is widely applied in commercial maize breeding programs because of its time-saving and cost-reducing features. The production of maize haploids primarily depends on the use of Stock6-derived haploid inducer lines. Although the gene underlying haploid induction, MTL/ZmPLA1/NLD, was cloned recently, the mechanism of haploid induction is still unknown. Hetero-fertilization can occur via a single fertilization, which provides a means to investigate single-fertilization events by studying the hetero-fertilization phenomenon. In this study, we found that the hetero-fertilization rate increased significantly when female maize lines were first individually crossed with pollen from the inducer CAU5 in dual-pollination experiments 4 h before a second pollination with common lines. We also examined embryogenesis during haploid induction by confocal laser-scanning microscopy and observed single-fertilized ovules, indicating that single fertilization occurred during haploid induction. We therefore postulate that both single fertilization and chromosome elimination contribute to haploid induction in maize. We also propose a scheme for the formation of hetero-fertilized and haploid kernels. Our results provide an efficient approach to identify hetero-fertilized kernels for research on interactions between embryo and endosperm.

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