文献类型： 外文期刊

作者： Ma, Lin ¹ ; Zhang, Yongchao ² ; Wen, Hongyu ¹ ; Liu, Wenhui ² ; Zhou, Yu ³ ; Wang, Xuemin ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

2.Qinghai Univ, Qinghai Acad Anim Sci & Vet Med, Key Lab Super Forage Germplasm Qinghai Tibetan Pl, Xining 810016, Peoples R China

3.Chongqing Acad Agr Sci, Inst Characterist Crops Res, Chongqing 402160, Peoples R China

关键词： alfalfa; MsD14; strigolactone; shoot branching; forage biomass

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 7 期

页码：

收录情况： SCI

摘要： Branching is one of the key determinants of plant architecture that dramatically affects crop yield. As alfalfa is the most important forage crop, understanding the genetic basis of branching in this plant can facilitate breeding for a high biomass yield. In this study, we characterized the strigolactone receptor gene MsD14 in alfalfa and demonstrated that MsD14 was predominantly expressed in flowers, roots, and seedpods. Furthermore, we found that MsD14 expression could significantly respond to strigolactone in alfalfa seedlings, and its protein was located in the nucleus, cytoplasm, and cytomembrane. Most importantly, transformation assays demonstrated that silencing of MsD14 in alfalfa resulted in increased shoot branching and forage biomass. Significantly, MsD14 could physically interact with AtMAX2 and MsMAX2 in the presence of strigolactone, suggesting a similarity between MsD14 and AtD14. Together, our results revealed the conserved D14-MAX2 module in alfalfa branching regulation and provided candidate genes for alfalfa high-yield molecular breeding.