Epigallocatechin Gallate (EGCG) Inhibited the Alv-J-Induced Apoptosis in Df-1 Cells by Inactivation of Nuclear Factor kappa b Pathway
文献类型： 外文期刊
作者： Zhang, Y. 1 ; Yang, H. 1 ; Wu, X. 1 ; Deng, M. 1 ; Li, Z. 1 ; Xu, Z. 1 ;
作者机构： 1.Chongqing Acad Agr Sci, Tea Res Inst, Chongqing 402160, Yongchuan, Peoples R China
关键词： ALV-J; EGCG; NF-kappa B; DF-1 cell; apoptosis
期刊名称：BRAZILIAN JOURNAL OF POULTRY SCIENCE （ 影响因子：1.1； 五年影响因子：1.2 ）
ISSN： 1516-635X
年卷期： 2019 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Avian leukosis virus subgroup J (ALV-J), a member of the retroviridae family, can infect both broilers and layers and induce a spectrum of different neoplasms, resulting in serious economic losses in poultry production. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has demonstrated remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems and epidemiological studies. To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, DF-1 cells were treated with different EGCG concentrations (0, 5, 10, 20 and 40 mu g/mL), and their antiviral effects were examined at different time points (0, 24, 48, 72 and 96 h) using a variety of assays. EGCG alleviated the ALV-J-induced apoptosis in a dose-dependent manner. Because high concentrations (20 and 40 mu g/mL) inhibited DF-1 cell growth, and low concentration (5 mu g/ mL) did not suppress the ALV-J virus, 10 mu g/mL was the most appropriate concentration. After 96 h of incubation, 10 mu g/mL EGCG improved the ALV-J-triggered suppression of the nuclear transcription factor system by enhancing cytoplasmic NF-kappa B p50/p65 expression and inhibiting nuclear NF-kappa B p50/p65 expression, resulting in decreased cell apoptosis. These results demonstrated that EGCG inhibited ALV-J-induced apoptosis in DF-1 cells in a dose-dependent manner via the NE-kappa B signaling pathway, and that 10 mu g/mL EGCG is the optimal concentration, which may be useful for therapeutic drug design.
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